Dataset Information

Genomic Characterization of a Carbapenemase-Producing, Extensively Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess Patient.

ABSTRACT: We describe an extensively drug-resistant Klebsiella michiganensis strain, Kmfe267, which was originally isolated from a renal abscess patient. The strain carries the blaNDM-5 gene, which encodes a New Delhi metallo-β-lactamase. The complete genome of the strain contains a 5.9-Mb chromosome and 5 plasmids.

SUBMITTER: Hu LM

PROVIDER: S-EPMC9753623 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Genomic Characterization of a Carbapenemase-Producing, Extensively Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess Patient.

Hu Li-Miao LM Zhang Xiao-Tuan XT Zeng Xi X Chen Yajun Y Liu Logen L Li Guo-Qing GQ

Microbiology resource announcements 20221102 12

We describe an extensively drug-resistant Klebsiella michiganensis strain, Kmfe267, which was originally isolated from a renal abscess patient. The strain carries the blaNDM-5 gene, which encodes a New Delhi metallo-β-lactamase. The complete genome of the strain contains a 5.9-Mb chromosome and 5 plasmids. ...[more]

PMID: 36321904

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Project description:BackgroundCarbapenemase-producing Klebsiella pneumoniae (CP-Kp) poses distinct clinical challenges due to extensively drug resistant (XDR) phenotype, and sequence type (ST) 11 is the most dominant blaKPC-2-bearing CP-Kp clone in China. The purpose of this current retrospective study was to explore the genetic factors associated with the success of XDR CP-Kp ST11 strains circulated in the intensive care unit (ICU) of a Chinese tertiary hospital.MethodsSix ST11 XDR CP-Kp strains were identified between May and December 2014 and validated by minimum inhibitory concentration examination, polymerase chain reaction, and pyrosequencing. The six ST11 XDR CP-Kp, as well as three multi-drug resistant (MDR) and four susceptible strains, were sequenced using single-molecule real-time method. Comprehensively structural and functional analysis based on comparative genomics was performed to identify genomic characteristics of the XDR ST11 CP-Kp strains.ResultsWe found that ST11 XDR blaKPC-2-bearing CP-Kp strains isolated from inpatients spread in the ICU of the hospital. Functionally, genes associated with information storage and processing of the ST11 XDR CP-Kp strains were more abundant than those of MDR and susceptible strains, especially genes correlative with mobile genetic elements (MGEs) such as transposons and prophages. Structurally, eleven large-scale genetic regions taken for the unique genome in these ST11 XDR CP-Kp strains were identified as MGEs including transposons, integrons, prophages, genomic islands, and integrative and conjugative elements. Three of them were located on plasmids and eight on chromosomes; five of them were with antimicrobial resistance genes and eight with adaptation associated genes. Notably, a new blaKPC-2-bearing ΔΔTn1721-blaKPC-2 transposon, probably transposed and truncated from ΔTn1721-blaKPC-2 by IS903D and ISKpn8, was identified in all six ST11 XDR CP-Kp strains.ConclusionOur findings suggested that together with clonal spread, MGEs identified uniquely in the ST11 XDR CP-Kp strains might contribute to their formidable adaptability, which facilitated their widespread dissemination in hospital.

Dataset Information

Genomic Characterization of a Carbapenemase-Producing, Extensively Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess Patient.

Publications

Genomic Characterization of a Carbapenemase-Producing, Extensively Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess Patient.

Similar Datasets

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