Dataset Information

Whole-Genome Sequences of Antibiotic-Resistant Trueperella pyogenes Isolates from Surgical Site Infections in Dairy Cows in Switzerland.

ABSTRACT: The complete genome sequence of four Trueperella pyogenes isolated from cattle surgical site infections in Switzerland was determined using hybrid assembly of Oxford Nanopore and Illumina reads. Genes conferring resistance to tetracyclines [tet(W)], sulfonamides (sul1), chloramphenicol (cmx), streptomycin/spectinomycin (aadA1), and quaternary ammonium compounds (qacEΔ1) were identified on different chromosomal elements.

SUBMITTER: Marchionatti E

PROVIDER: S-EPMC9753664 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Whole-Genome Sequences of Antibiotic-Resistant Trueperella pyogenes Isolates from Surgical Site Infections in Dairy Cows in Switzerland.

Marchionatti Emma E Perreten Vincent V

Microbiology resource announcements 20221115 12

The complete genome sequence of four Trueperella pyogenes isolated from cattle surgical site infections in Switzerland was determined using hybrid assembly of Oxford Nanopore and Illumina reads. Genes conferring resistance to tetracyclines [tet(W)], sulfonamides (sul1), chloramphenicol (cmx), streptomycin/spectinomycin (aadA1), and quaternary ammonium compounds (qacEΔ1) were identified on different chromosomal elements. ...[more]

PMID: 36377956

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Project description:Trueperella pyogenes (T. pyogenes) is an important opportunistic animal pathogen that causes huge economic losses to the animal husbandry industry. The emergence of bacterial resistance and the unsatisfactory effect of the vaccine have prompted investigators to explore alternative strategies for controlling T. pyogenes infection. Due to the ability of phages to kill multidrug-resistant bacteria, the use of phage therapy to combat multidrug-resistant bacterial infections has attracted attention. In this study, a T. pyogenes phage, vB-ApyS-JF1 (JF1), was isolated from sewage samples, and its whole genome and biological characteristics were elucidated. Moreover, the protective effect of phage JF1 on a mouse bacteremic model caused by T. pyogenes was studied. JF1 harbors a double-stranded DNA genome with a length of 90,130 bp (30.57% G + C). The genome of JF1 lacked bacterial virulence-, antibiotic resistance- and lysogenesis-related genes. Moreover, the genome sequence of JF1 exhibited low coverage (<6%) with all published phages in the NCBI database, and a phylogenetic analysis of the terminase large subunits and capsid indicated that JF1 was evolutionarily distinct from known phages. In addition, JF1 was stable over a wide range of pH values (3 to 11) and temperatures (4 to 50°C) and exhibited strong lytic activity against T. pyogenes in vitro. In murine experiments, a single intraperitoneal administration of JF1 30 min post-inoculation provided 100% protection for mice against T. pyogenes infection. Compared to the phosphate-buffered saline (PBS) treatment group, JF1 significantly (P < 0.01) reduced the bacterial load in the blood and tissues of infected mice. Meanwhile, treatment with phage JF1 relieved the pathological symptoms observed in each tissue. Furthermore, the levels of the inflammatory cytokines tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and interleukin-6 (IL-6) in the blood of infected mice were significantly (P < 0.01) decreased in the phage-treated group. Taken together, these results indicate that phage JF1 demonstrated great potential as an alternative therapeutic treatment against T. pyogenes infection.

Project description:In this study we evaluate the efficacy of five vaccine formulations containing different combinations of proteins (FimH; leukotoxin, LKT; and pyolysin, PLO) and/or inactivated whole cells (Escherichia coli, Fusobacterium necrophorum, and Trueperella pyogenes) in preventing postpartum uterine diseases. Inactivated whole cells were produced using two genetically distinct strains of each bacterial species (E. coli, F. necrophorum, and T. pyogenes). FimH and PLO subunits were produced using recombinant protein expression, and LKT was recovered from culturing a wild F. necrophorum strain. Three subcutaneous vaccines were formulated: Vaccine 1 was composed of inactivated bacterial whole cells and proteins; Vaccine 2 was composed of proteins only; and Vaccine 3 was composed of inactivated bacterial whole cells only. Two intravaginal vaccines were formulated: Vaccine 4 was composed of inactivated bacterial whole cells and proteins; and Vaccine 5 was composed of PLO and LKT. To evaluate vaccine efficacy, a randomized clinical trial was conducted at a commercial dairy farm; 371 spring heifers were allocated randomly into one of six different treatments groups: control, Vaccine 1, Vaccine 2, Vaccine 3, Vaccine 4 and Vaccine 5. Late pregnant heifers assigned to one of the vaccine groups were each vaccinated twice: at 230 and 260 days of pregnancy. When vaccines were evaluated grouped as subcutaneous and intravaginal, the subcutaneous ones were found to significantly reduce the incidence of puerperal metritis. Additionally, subcutaneous vaccination significantly reduced rectal temperature at 6±1 days in milk. Reproduction was improved for cows that received subcutaneous vaccines. In general, vaccination induced a significant increase in serum IgG titers against all antigens, with subcutaneous vaccination again being more effective. In conclusion, subcutaneous vaccination with inactivated bacterial components and/or protein subunits of E. coli, F. necrophorum and T. pyogenes can prevent puerperal metritis during the first lactation of dairy cows, leading to improved reproduction.

Dataset Information

Whole-Genome Sequences of Antibiotic-Resistant Trueperella pyogenes Isolates from Surgical Site Infections in Dairy Cows in Switzerland.

Publications

Whole-Genome Sequences of Antibiotic-Resistant Trueperella pyogenes Isolates from Surgical Site Infections in Dairy Cows in Switzerland.

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