MALDI-TOF mass spectrometry profiling of bovine skim milk for subclinical mastitis detection.

ABSTRACT:

Introduction

Mastitis is one of most impacting health issues in bovine dairy farming that reduces milk yield and quality, leading to important economic losses. Subclinical forms of the disease are routinely monitored through the measurement of somatic cell count (SCC) and microbiological tests. However, their identification can be tricky, reducing the possibilities of early treatments. In this study, a MALDI-TOF mass spectrometry approach was applied to milk samples collected from cows classified according to the SCC, to identify differences in polypeptide/protein profiles.

Materials and methods

Twenty-nine raw milk samples with SCC >200,000 cell/ml (group H) and 91 samples with SCC lower than 200,000 (group L) were randomly collected from 12 dairy farms. Spectral profiles f

SUBMITTER: Cuccato M 

PROVIDER: S-EPMC9753775 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature