4-1BB and cytokines trigger human NK, γδ T, and CD8⁺ T cell proliferation and activation, but are not required for their effector functions.

ABSTRACT:

Introduction

This study was designed to compare the costimulatory molecules and cytokines required to trigger the proliferation and activation of natural killer (NK), γδ T, and CD8⁺ T cells, and gain in-depth insight into the mechanisms shifting tolerance to immunity.

Methods

K562-derived artificial antigen-presenting cells (aAPCs); that is, K562 forced to express CD86 and 4-1BBL costimulatory receptors, in the presence of cytokines, were used to mimic dendritic cells (DCs) and provide signals to support the proliferation and activation of NK, γδ T, and CD8⁺ T cells.

Results

Three signals are required to trigger optimal proliferation in MART-1-specific CD8⁺ T cells: activation of T-cell receptors (TCRs) by the major histocompatibility

SUBMITTER: Vidard L 

PROVIDER: S-EPMC9753824 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature