Advances in the Computational Design of Small-Molecule-Controlled Protein-Based Circuits for Synthetic Biology.

ABSTRACT: Synthetic biology approaches living systems with an engineering perspective and promises to deliver solutions to global challenges in healthcare and sustainability. A critical component is the design of biomolecular circuits with programmable input-output behaviors. Such circuits typically rely on a sensor module that recognizes molecular inputs, which is coupled to a functional output via protein-level circuits or regulating the expression of a target gene. While gene expression outputs can be customized relatively easily by exchanging the target genes, sensing new inputs is a major limitation. There is a limited repertoire of sensors found in nature, and there are often difficulties with interfacing them with engineered circuits. Computational protein design could be a key enabling techn

SUBMITTER: Kretschmer S 

PROVIDER: S-EPMC9754107 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature