Single-cell RNA sequencing reveals the Muller subtypes and inner blood-retinal barrier regulatory network in early diabetic retinopathy.

ABSTRACT: As the basic pathological changes of diabetic retinopathy (DR), the destruction of the blood-retina barrier (BRB) and vascular leakage have attracted extensive attention. Without timely intervention, BRB damage will eventually lead to serious visual impairment. However, due to the delicate structure and complex function of the BRB, the mechanism underlying damage to the BRB in DR has not been fully clarified. Here, we used single-cell RNA sequencing (RNA-seq) technology to analyze 35,910 cells from the retina of healthy and streptozotocin (STZ)-induced diabetic rats, focusing on the degeneration of the main cells constituting the rat BRB in DR and the new definition of two subpopulations of Müller cells at the cell level, Ctxn3 ⁺Müller and Ctxn3 ^-Müller

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9754943 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature