Tuberculosis detection in nonhuman primates is enhanced by use of testing algorithms that include an interferon-γ release assay.

ABSTRACT:

Objective

To develop a testing algorithm that incorporates multiple assays to evaluate host cellular and humoral immunity and antigen detection concerning Mycobacterium tuberculosis complex (MTBC) infection in captive nonhuman primates.

Animals

Cohorts of captive-bred and wild-caught macaques from 5 different geographic regions.

Procedures

Macaques were tested for MTBC infection by use of a γ interferon tuberculosis (GIFT) assay, an interferon-γ release assay, and other assays. In the first 2 cohorts (n = 15 and 181), initial validation of the GIFT assay was performed by use of experimentally infected and unexposed control macaques. In the next 3 cohorts (n = 59, 42, and 11), results were obtained for opportunistically collected samples from macaques exposed during sp

SUBMITTER: Yee JL 

PROVIDER: S-EPMC9754947 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature