Performance evaluation and clinical validation of optimized nucleotide MALDI-TOF-MS for mycobacterial identification.

ABSTRACT:

Objective

To evaluate the performance and validate the diagnostic value of a nucleotide matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) with the analysis process optimized in identification of mycobacterium species.

Methods

The optimized analysis process was used for mycobacterial identification in the nucleic MALDI-TOF-MS. 108 samples were used for assessing the performance of nucleic MALDI-TOF-MS, including 25 reference standards, 37 clinical isolates, 37 BALF, and 9 plasmids. The BALF of 38 patients suspected of pulmonary mycobacterial infection was collected for validation. Clinical etiological diagnosis was used as the gold standard to evaluate the diagnostic value of nucleotide MALDI-TOF-MS.

Results

The sensitivity, specificity,

SUBMITTER: Li B 

PROVIDER: S-EPMC9755490 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature