Real-time reverse transcription recombinase polymerase amplification for rapid detection of murine hepatitis virus.

ABSTRACT: Murine hepatitis virus (MHV) is a highly infectious murine coronavirus that has a high potential for causing harm to host animals. This study aimed to develop a real-time reverse transcription recombinase polymerase amplification (RT-RPA) method for rapid detection of MHV in laboratory mice.

Methods

Specific primers and probes for RT-RPA assay were designed targeting the conserved region in the M gene of the MHV reference strain (accession no. FJ6647223) according to the TwistDx manual instructions. The specificity, sensitivity, and reproducibility of the RT-RPA method were evaluated and compared with those of the standard RT-qPCR method. The clinical applicability of this assay was evaluated using 68 field samples.

Results

Amplification using the newly developed RT-RPA assay

SUBMITTER: Wang X 

PROVIDER: S-EPMC9755729 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature