MicroRNA-375 inhibits laryngeal squamous cell carcinoma progression via targeting CST1.

ABSTRACT:

Objective

This study aims to explore the effect and mechanism of miR-375 in Laryngeal Squamous Cell Carcinoma (LSCC) cell progression.

Methods

LSCC cells (LSC-1 and TU177) were transfected with miR-375-mimic, miR-375-inhibitor or miR-375-mimic+oe-CST1. The expression of miR-375, CST1, MMP-2, and MMP-9 was measured. The effect of miR-375-mimic, miR-375-inhibitor or miR-375-mimic+oe-CST1 on cell biological functions, including cell proliferation, migration, invasion, and apoptosis, was also assessed. The potential relationship between CST1 and miR-375 was predicted by Jefferson software and validated by dual luciferase reporter gene assay.

Results

Downregulated miR-375 expression was found in LSCC cells. Overexpression of miR-375 inhibited the viability and migration an

SUBMITTER: Dai F 

PROVIDER: S-EPMC9756075 | biostudies-literature | 2022 Nov-Dec

REPOSITORIES: biostudies-literature