Bacterial chemotaxis in static gradients quantified in a biopolymer membrane-integrated microfluidic platform.

ABSTRACT: Chemotaxis is a fundamental bacterial response mechanism to changes in chemical gradients of specific molecules known as chemoattractant or chemorepellent. The advancement of biological platforms for bacterial chemotaxis research is of significant interest for a wide range of biological and environmental studies. Many microfluidic devices have been developed for its study, but challenges still remain that can obscure analysis. For example, cell migration can be compromised by flow-induced shear stress, and bacterial motility can be impaired by nonspecific cell adhesion to microchannels. Also, devices can be complicated, expensive, and hard to assemble. We address these issues with a three-channel microfluidic platform integrated with natural biopolymer membranes that are assembled in si

SUBMITTER: Hu P 

PROVIDER: S-EPMC9756273 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature