The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii.

ABSTRACT:

Background

The budding yeast Komagataella phaffii (Pichia pastoris) is widely employed to secrete proteins of academic and industrial interest. For secretory proteins, signal peptides are the sorting signal to direct proteins from cytosol to extracellular matrix, and their secretion efficiency directly impacts the yields of the targeted proteins in fermentation broth. Although the α-mating factor (MF) secretion signal from S. cerevisiae, the most common and widely used signal sequence for protein secretion, works in most cases, limitation exists as some proteins cannot be secreted efficiently. As the optimal choice of secretion signals is often protein specific, more secretion signals need to be developed to augment protein expression levels in K. phaffii.

Results

In this st

SUBMITTER: Zou C 

PROVIDER: S-EPMC9756452 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature