Oncogenic KRAS alters splicing factor phosphorylation and alternative splicing in lung cancer.

ABSTRACT:

Background

Alternative RNA splicing is widely dysregulated in cancers including lung adenocarcinoma, where aberrant splicing events are frequently caused by somatic splice site mutations or somatic mutations of splicing factor genes. However, the majority of mis-splicing in cancers is unexplained by these known mechanisms. We hypothesize that the aberrant Ras signaling characteristic of lung cancers plays a role in promoting the alternative splicing observed in tumors.

Methods

We recently performed transcriptome and proteome profiling of human lung epithelial cells ectopically expressing oncogenic KRAS and another cancer-associated Ras GTPase, RIT1. Unbiased analysis of phosphoproteome data identified altered splicing factor phosphorylation in KRAS-mutant cells, so we perfor

SUBMITTER: Lo A 

PROVIDER: S-EPMC9756471 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature