Transcorneal but not transpalpebral electrical stimulation disrupts mucin homeostasis of the ocular surface.

ABSTRACT:

Purpose

Transcorneal electrical stimulation (TcES) is increasingly applied as a therapy for preserving and improving vision in retinal neurodegenerative and ischemic disorders. However, a common complaint about TcES is its induction of eye pain and dryness in the clinic, while the mechanisms remain unknown.

Method

TcES or transpalpebral ES (TpES) was conducted in C57BL6j mice for 14 days. The contralateral eyes were used as non-stimulated controls. Levels of intracellular [Ca²⁺] ([Ca²⁺]_i) were assessed by Fura-2AM. The conductance resistances of the eye under various ES conditions were measured in vivo by an oscilloscope.

Results

Although TcES did not affect tear production, it significantly induced damage to the ocular surface, as rev

SUBMITTER: Yang M 

PROVIDER: S-EPMC9756492 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature