Efficient generation of epigenetic disease model mice by epigenome editing using the piggyBac transposon system.

ABSTRACT:

Background

Epigenome-edited animal models enable direct demonstration of disease causing epigenetic mutations. Transgenic (TG) mice stably expressing epigenome-editing factors exhibit dramatic and stable changes in target epigenome modifications. Successful germline transmission of a transgene from founder mice to offspring will yield a sufficient number of epigenome-edited mice for phenotypic analysis; however, if the epigenetic mutation has a detrimental phenotypic effect, it can become difficult to obtain the next generation of animals. In this case, the phenotype of founder mice must be analyzed directly. Unfortunately, current TG mouse production efficiency (TG founders per pups born) is relatively low, and improvements would increase the versatility of this technology.

Res

SUBMITTER: Horii T 

PROVIDER: S-EPMC9756621 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature