Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesity.

ABSTRACT:

Purpose

To study whether physical exercise can effectively ameliorate obesity-induced abnormalities in male fertility and provide a new perspective on the role of small noncoding RNAs in spermatogenesis in obese male mice.

Methods

In this study, four-week-old C57/Bl6 male mice were randomly allocated to receive a control diet, a high-fat diet or physical exercise intervention for 40 weeks. Purified round spermatids and spermatozoa were obtained after intervention. Sperm motility, concentration, the ability of the sperm to undergo capacitation and acrosome reaction were assessed. Small RNA sequencing was conducted on round spermatids and spermatozoa. The small noncoding RNAs expression pattern was systematically analyzed.

Results

The spermatozoa concentration and perce

SUBMITTER: Lin T 

PROVIDER: S-EPMC9756842 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature