Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma.

ABSTRACT:

Background

Lung adenocarcinoma (LUAD) is a highly malignant tumor with a very low five-year survival rate. In this study, we aimed to identify differentially expressed long-chain non-coding RNA (lncRNAs) and mRNAs from benign and malignant pleural effusion exosomes.

Methods

We used gene microassay and quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) to detect and verify differentially expressed mRNAs and lncRNAs in benign and malignant pleural effusion exosomes. Gene Ontology (GO) functional significance and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway significance enrichment analyses were performed to identify the difference in biological processes and functions between different mRNAs. We selected the lncRNA ZBED5-AS1 with an upreg

SUBMITTER: Huang X 

PROVIDER: S-EPMC9756994 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature