Deciphering the map of METTL14-mediated lncRNA m6A modification at the transcriptome-wide level in breast cancer.

ABSTRACT:

Background

Emerging studies have demonstrated the critical role of RNA m6A methylation in tumor progression, whereas lncRNA m6A modification profiles in breast cancer remain largely unknown. Our previous study has shown that METTL14 accelerates breast cancer migration and invasion in an m6A-dependent manner, making it critical to analyze METTL14-mediated m6A modification at a transcriptome-wide scale in breast cancer.

Methods

Here, we performed MeRIP-seq analysis in METTL14 overexpressed and control MDA-MB-231 cells. Conjoint analysis of MeRIP-seq and RNA-seq data was used to select lncRNAs with m6A methylation and differential expression. Finally, the screened lncRNA was verified by MeRIP-PCR and its function was studied via transwell assay.

Results

Our results deter

SUBMITTER: Yi D 

PROVIDER: S-EPMC9757010 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature