DDX17 helicase promotes resolution of R-loop-mediated transcription-replication conflicts in human cells.

ABSTRACT: R-loops are three-stranded nucleic acid structures composed of an RNA:DNA hybrid and displaced DNA strand. These structures can halt DNA replication when formed co-transcriptionally in the opposite orientation to replication fork progression. A recent study has shown that replication forks stalled by co-transcriptional R-loops can be restarted by a mechanism involving fork cleavage by MUS81 endonuclease, followed by ELL-dependent reactivation of transcription, and fork religation by the DNA ligase IV (LIG4)/XRCC4 complex. However, how R-loops are eliminated to allow the sequential restart of transcription and replication in this pathway remains elusive. Here, we identified the human DDX17 helicase as a factor that associates with R-loops and counteracts R-loop-mediated replication stress t

SUBMITTER: Boleslavska B 

PROVIDER: S-EPMC9757067 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature