Intracavitary adoptive transfer of IL-12 mRNA-engineered tumor-specific CD8⁺ T cells eradicates peritoneal metastases in mouse models.

ABSTRACT: Previous studies have shown that local delivery of tumor antigen-specific CD8⁺ T lymphocytes engineered to transiently express single-chain IL-12 mRNA is highly efficacious. Peritoneal dissemination of cancer is a frequent and often fatal patient condition usually diagnosed when the tumor burden is too large and hence uncontrollable with current treatment options. In this study, we have modeled intracavitary adoptive T cell therapy with OVA-specific OT-I T cells electroporated with IL-12 mRNA to treat B16-OVA and PANC02-OVA tumor spread in the peritoneal cavity. Tumor localization in the omentum and the effects of local T-cell encounter with the tumor antigens were monitored, the gene expression profile evaluated, and the phenotypic reprogramming of several immune subsets was ch

SUBMITTER: Di Trani CA 

PROVIDER: S-EPMC9757485 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature