An organoid library of salivary gland tumors reveals subtype-specific characteristics and biomarkers.

ABSTRACT:

Background

Salivary gland tumors (SGTs) include a large group of rare neoplasms in the head and neck region, and the heterogeneous and overlapping features among the subtypes frequently make diagnostic difficulties. There is an urgent need to understand the cellular mechanisms underlying the heterogeneity and overlap among the subtypes, and explore the subtype-specific diagnostic biomarkers.

Methods

The tumor tissue and the adjacent normal tissue from the 6 most common types of SGTs were processed for organoid culture which only maintained tumor epithelial cells. Organoids were histologically evaluated based on phenotype markers, followed by transcriptional profiling using RNA-sequencing. The transcriptomic similarities and differences among the subtypes were analyzed by sub

SUBMITTER: Wang B 

PROVIDER: S-EPMC9758872 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature