Alveolus analysis: a web browser-based tool to analyze lung intravital microscopy.

ABSTRACT:

Background

Acute lung injury and the acute respiratory distress syndrome are characterized by pulmonary inflammation, reduced endothelial barrier integrity and filling of the alveolar space with protein rich edema fluid and infiltrating leukocytes. Animal models are critical to uncovering the pathologic mechanisms of this devastating syndrome. Intravital imaging of the intact lung via two-photon intravital microscopy has proven a valuable method to investigate lung injury in small rodent models through characterization of inflammatory cells and vascular changes in real time. However, respiratory motion complicates the analysis of these time series images and requires selective data extraction to stabilize the image. Consequently, analysis of individual alveoli may not provide a com

SUBMITTER: Politowicz AL 

PROVIDER: S-EPMC9759058 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature