Transcriptomics of bronchoalveolar lavage cells identifies new molecular endotypes of sarcoidosis.

ABSTRACT:

Background

Sarcoidosis is a multisystem granulomatous disease of unknown origin with a variable and often unpredictable course and pattern of organ involvement. In this study we sought to identify specific bronchoalveolar lavage (BAL) cell gene expression patterns indicative of distinct disease phenotypic traits.

Methods

RNA sequencing by Ion Torrent Proton was performed on BAL cells obtained from 215 well-characterised patients with pulmonary sarcoidosis enrolled in the multicentre Genomic Research in Alpha-1 Antitrypsin Deficiency and Sarcoidosis (GRADS) study. Weighted gene co-expression network analysis and nonparametric statistics were used to analyse genome-wide BAL transcriptome. Validation of results was performed using a microarray expression dataset of an independe

SUBMITTER: Vukmirovic M 

PROVIDER: S-EPMC9759791 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature