Enabling online determination of the size-dependent RNA content of lipid nanoparticle-based RNA formulations.

ABSTRACT: The potential of lipid nanoparticles (LNPs) as nucleic acid delivery vehicles has been demonstrated in recent years, culminating in the emergency use approval of LNP-based mRNA SARS-CoV-2 vaccines in late 2020. The determination of RNA content relative to LNP size can be important to the understanding of efficacy and adverse effects. This work presents the first description of a facile and rapid analytical method for online, size-dependent RNA payload distribution measurement using data from multi-angle light scattering, ultraviolet and refractive index detectors following separation of the LNPs by size-exclusion chromatography. The analysis was validated by size-based fractionation of the LNPs with subsequent offline analysis of the fractions. Four LNPs formulated with different PEG-lipid

SUBMITTER: Jia X 

PROVIDER: S-EPMC9760250 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature