Repeated dosing improves oncolytic rhabdovirus therapy in mice via interactions with intravascular monocytes.

ABSTRACT: There is debate in the field of oncolytic virus (OV) therapy, whether a single viral dose, or multiple administrations, is better for tumor control. Using intravital microscopy, we describe the fate of vesicular stomatitis virus (VSV) delivered systemically as a first or a second dose. Following primary administration, VSV binds to the endothelium, initiates tumor infection and activates a proinflammatory response. This initial OV dose induces neutrophil migration into the tumor and limits viral replication. OV administered as a second dose fails to infect the tumor and is captured by intravascular monocytes. Despite a lack of direct infection, this second viral dose, in a monocyte-dependent fashion, enhances and sustains infection by the first viral dose, promotes CD8 T cell recruitment,

SUBMITTER: Naumenko V 

PROVIDER: S-EPMC9761050 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature