Novel protein contact points among TP53 and minichromosome maintenance complex proteins 2, 3, and 5.

ABSTRACT:

Objective

Identify protein contact points between TP53 and minichromosome maintenance (MCM) complex proteins 2, 3, and 5 with high resolution allowing for potential novel Cancer drug design.

Methods

A next-generation sequencing-based protein-protein interaction method developed in our laboratory called AVA-Seq was applied to a gold-standard human protein interaction set. Proteins including TP53, MCM2, MCM3, MCM5, HSP90AA1, PCNA, NOD1, and others were sheared and ligated into the AVA-Seq system. Protein-protein interactions were then identified in both mild and stringent selective conditions.

Results

Known interactions among MCM2, MCM3, and MCM5 were identified with the AVA-Seq system. The interacting regions detected between these three proteins overlap with the struc

SUBMITTER: Schaefer-Ramadan S 

PROVIDER: S-EPMC9761056 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature