Magnetic activation of TREK1 triggers stress signalling and regulates neuronal branching in SH-SY5Y cells.

ABSTRACT: TWIK-related K⁺ 1 (TREK1) is a potassium channel expressed in the nervous system with multiple functions including neurotransmission and is a prime pharmacological target for neurological disorders. TREK1 gating is controlled by a wide range of external stimuli including mechanical forces. Previous work has demonstrated that TREK1 can be mechano-activated using magnetic nanoparticles (MNP) functionalised with antibodies targeted to TREK1 channels. Once the MNP are bound, external dynamic magnetic fields are used to generate forces on the TREK channel. This approach has been shown to drive cell differentiation in cells from multiple tissues. In this work we investigated the effect of MNP-mediated TREK1 mechano-activation on early stress response pathways along with the differenti

SUBMITTER: Rotherham M 

PROVIDER: S-EPMC9761330 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature