Development of a thiostrepton-free system for stable production of PLD in Streptomyces lividans SBT5.

ABSTRACT:

Background

Phospholipase D (PLD) is highly valuable in the food and medicine industries, where it is used to convert low-cost phosphatidylcholine into high-value phospholipids (PLs). Despite being overexpressed in Streptomyces, PLD production requires expensive thiostrepton feeding during fermentation, limiting its industrialization. To address this issue, we propose a new thiostrepton-free system.

Results

We developed a system using a combinatorial strategy containing the constitutive promoter kasOp* and PLD G215S mutation fused to a signal peptide sigcin of Streptoverticillium cinnamoneum pld. To find a candidate vector, we first expressed PLD using the integrative vector pSET152 and then built three autonomously replicating vectors by substituting Streptomyces replicons t

SUBMITTER: Wang J 

PROVIDER: S-EPMC9761944 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature