WHO cone bioassay boards with or without holes: relevance for bioassay outcomes in long-lasting insecticidal net studies.

ABSTRACT:

Background

The World Health Organization (WHO) cone bioassay is a key method used to evaluate the bioefficacy of long-lasting insecticidal nets (LLINs) used for malaria control. These tests also play an important role in LLIN product prequalification and longitudinal monitoring. Standardization of these assays is therefore important. While many parameters for WHO cone bioassays are defined in the respective WHO guidelines, others are not. One of these undefined parameters is the exact configuration of the bioassay boards. In cone bioassays, LLIN samples are pinned onto a bioassay board for testing. Anecdotal evidence suggests that bioassay boards with holes behind the LLIN samples lead to greater exposure to insecticide, as the mosquitoes are 'forced to stand on the net material'.

SUBMITTER: Koinari M 

PROVIDER: S-EPMC9762087 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature