Tear Film miRNAs and Their Association With Human Dry Eye Disease.

ABSTRACT:

Purpose

miRNAs can regulate inflammatory pathways. The purpose of this work was to determine if inflammatory-related tear film miRNAs are associated with extracellular vesicles (EVs) in human non-Sjögren's Syndrome dry eye disease (DED) participants.

Methods

Five DED and 5 non-DED human participants were recruited. Tears samples were collected by washing the ocular surface of both eyes with phosphate buffered saline, pooling samples from the right and left eyes, and purifying EVs from the samples with a polyethylene glycol (PEG) 8000 precipitation procedure. Samples were directly analyzed via ELISA or transmission electron microscopy (TEM), or RNA was isolated first from the EVs and evaluated with RNA-Seq.

Results

EVs were identified in the tear film of both groups us

SUBMITTER: Pucker AD 

PROVIDER: S-EPMC9762462 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature