Will previous palliative surgery for congenital heart disease be detrimental to subsequent pig heart xenotransplantation?

ABSTRACT:

Introduction

Pig heart xenotransplantation might act as a bridge in infants with complex congenital heart disease (CHD) until a deceased human donor heart becomes available. Infants develop antibodies to wild-type (WT, i.e., genetically-unmodified) pig cells, but rarely to cells in which expression of the 3 known carbohydrate xenoantigens has been deleted by genetic engineering (triple-knockout [TKO] pigs). Our objective was to test sera from children who had undergone palliative surgery for complex CHD (and who potentially might need a pig heart transplant) to determine whether they had serum cytotoxic antibodies against TKO pig cells.

Methods

Sera were obtained from children with CHD undergoing Glenn or Fontan operation (n = 14) and healthy adults (n = 8, as controls). All

SUBMITTER: Oscherwitz M 

PROVIDER: S-EPMC9762890 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature