A novel technique for the identification of CpG islands exhibiting altered methylation patterns (ICEAMP).

ABSTRACT: Aberrant CpG methylation changes occurring during tumour progression include the loss (hypomethylation) and gain (hypermethylation) of methyl groups. Techniques currently available for examining such changes either require selection of a region, then examination of methylation changes, or utilise methylation-sensitive restriction enzymes to identify an alteration. We describe here a novel method that identifies genomic regions as a consequence of altered methylation during tumourigenesis. A methyl-CpG binding domain column isolates methylated GC-rich sequences from both tumours and surrounding normal tissue. Subsequent subtractive hybridisation removes sequences common to both, leaving only methylated sequences unique to the tumour. Libraries of sequences generated using DNA derived from a

SUBMITTER: Brock GJ 

PROVIDER: S-EPMC97633 | biostudies-literature | 2001 Dec

REPOSITORIES: biostudies-literature