Circulating methylation level of HTR2A is associated with inflammation and disease activity in rheumatoid arthritis.

ABSTRACT:

Objectives

HTR2A is previously identified as a susceptibility gene for rheumatoid arthritis (RA). In this study, we performed the association analysis between DNA methylation of HTR2A with RA within peripheral blood samples.

Methods

We enrolled peripheral blood samples from 235 patients with RA, 30 osteoarthritis (OA) patients, and 30 healthy controls. The DNA methylation levels of about 218 bp from chr13: 46898190 to chr13: 46897973 (GRCh38/hg38) around HTR2A cg15692052 from patients were analyzed by targeted methylation sequencing.

Results

We measured methylation status for 7 CpGs in the promoter region of HTR2A and obseved overall methylation status are signficantly increased in RA compared with normal inviduals (FDR= 9.05 x 10

SUBMITTER: Zhao J 

PROVIDER: S-EPMC9763304 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature