Identification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma.
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ABSTRACT:
SUBMITTER: Li MM
PROVIDER: S-EPMC9763774 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Publications
Identification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma.
Li Mei-Mei MM Kong Fan-En FE Li Guang-Meng GM He Yi-Ti YT Zhang Xiao-Feng XF Zhang Cheng-Yang CY Liang Jie-Kai JK Guan Xin-Yuan XY Ma Ning-Fang NF Xie Mao-Bin MB Liu Ming M
STAR protocols 20221209 4
Here, we present a detailed protocol for the identification of potential oncofetal targets for hepatocellular carcinoma (HCC) patients through a hepatocyte differentiation model and a sorafenib refractory cell-line-derived xenograft model. We describe the procedures of tumor sphere formation, organoid generation, and subcutaneous tumor formation for functional studies. We then detail the procedures of immunohistochemistry and immunofluorescence for examination of changes in lineage-specific mark ...[more]
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