Role of the membrane anchor in the regulation of Lck activity.

ABSTRACT: Theoretical work suggests that collective spatiotemporal behaviour of integral membrane proteins (IMPs) should be modulated by boundary lipids sheathing their membrane anchors. Here, we show evidence for this prediction whilst investigating the mechanism for maintaining a steady amount of the active form of IMP Lck kinase (Lck_A) by Lck trans-autophosphorylation regulated by the phosphatase CD45. We used super-resolution microscopy, flow cytometry, and pharmacological and genetic perturbation to gain insight into the spatiotemporal context of this process. We found that Lck_A is generated exclusively at the plasma membrane, where CD45 maintains it in a ceaseless dynamic equilibrium with its unphosphorylated precursor. Steady Lck_A shows linear dependence, afte

SUBMITTER: Porciello N 

PROVIDER: S-EPMC9763865 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature