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Use of ubiquitous chromatin opening elements (UCOE) as tools to maintain transgene expression in biotechnology.

ABSTRACT: Amongst the most important outputs of the biopharmaceutical industry are recombinant proteins, many of which are produced by integrating transgenes into the genomes of mammalian cells. However, expression is highly variable and can be unstable during prolonged culture. This is often due to epigenetic mechanisms silencing the transgenes. To combat this problem, vectors have been engineered to include ubiquitous chromatin opening elements (UCOEs) that protect against silencing. Here, we recount the evidence that UCOEs can modify chromatin environments and benefit biomanufacturing.

SUBMITTER: Sizer RE

PROVIDER: S-EPMC9764128 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Use of ubiquitous chromatin opening elements (UCOE) as tools to maintain transgene expression in biotechnology.

Sizer Rebecca E RE White Robert J RJ

Computational and structural biotechnology journal 20221207

Amongst the most important outputs of the biopharmaceutical industry are recombinant proteins, many of which are produced by integrating transgenes into the genomes of mammalian cells. However, expression is highly variable and can be unstable during prolonged culture. This is often due to epigenetic mechanisms silencing the transgenes. To combat this problem, vectors have been engineered to include ubiquitous chromatin opening elements (UCOEs) that protect against silencing. Here, we recount th ...[more]

PMID: 36582439

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