Data-independent acquisition proteomics reveals circulating biomarkers of coronary chronic total occlusion in humans.

ABSTRACT:

Introduction

The pathophysiology of coronary chronic total occlusion (CTO) has not been fully elucidated.

Methods

In the present study, we aimed to investigate the potential plasma biomarkers associated with the pathophysiologic progression of CTO and identify protein dynamics in the plasma of CTO vessels immediately after successful revascularization. We quantitatively analyzed the plasma proteome profiles of controls (CON, n = 10) and patients with CTO pre- and post- percutaneous coronary intervention (PCI) (CTO, n = 10) by data-independent acquisition proteomics. We performed enzyme-linked immunosorbent assay (ELISA) to further confirm the common DEPs in the two-group comparisons (CON vs. CTO and CTO vs. CTO-PCI).

Results

A total of 1936 proteins with

SUBMITTER: Li J 

PROVIDER: S-EPMC9764215 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature