M⁷G-quant-seq: Quantitative Detection of RNA Internal N⁷-Methylguanosine.

ABSTRACT: Methods for the precise detection and quantification of RNA modifications are critical to uncover functional roles of diverse RNA modifications. The internal m⁷G modification in mammalian cytoplasmic tRNAs is known to affect tRNA function and impact embryonic stem cell self-renewal, tumorigenesis, cancer progression, and other cellular processes. Here, we introduce m⁷G-quant-seq, a quantitative method that accurately detects internal m⁷G sites in human cytoplasmic tRNAs at single-base resolution. The efficient chemical reduction and mild depurination can almost completely convert internal m⁷G sites into RNA abasic sites (AP sites). We demonstrate that RNA abasic sites induce a mixed variation pattern during reverse transcription, including G → A

SUBMITTER: Zhang LS 

PROVIDER: S-EPMC9764283 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature