Antibiotics Limit Adaptation of Drug-Resistant Staphylococcus aureus to Hypoxia.

ABSTRACT: Bacterial pathogens are confronted with a range of challenges at the site of infection, including exposure to antibiotic treatment and harsh physiological conditions, that can alter the fitness benefits and costs of acquiring antibiotic resistance. Here, we develop an experimental system to recapitulate resistance gene acquisition by Staphylococcus aureus and test how the subsequent evolution of the resistant bacterium is modulated by antibiotic treatment and oxygen levels, both of which are known to vary extensively at sites of infection. We show that acquiring tetracycline resistance was costly, reducing competitive growth against the isogenic strain without the resistance gene in the absence of the antibiotic, for S. aureus under hypoxic but not normoxic conditions. Treatment with tetra

SUBMITTER: Hull RC 

PROVIDER: S-EPMC9765076 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature