Redundancy in Citrate and cis-Aconitate Transport in Pseudomonas aeruginosa.

ABSTRACT: Tricarboxylates such as citrate are the preferred carbon sources for Pseudomonas aeruginosa, an opportunistic pathogen that causes chronic human infections. However, the membrane transport process for the tricarboxylic acid cycle intermediates citrate and cis-aconitate is poorly characterized. Transport is thought to be controlled by the TctDE two-component system, which mediates transcription of the putative major transporter OpdH. Here, we search for previously unidentified transporters of citrate and cis-aconitate using both protein homology and RNA sequencing approaches. We uncover new transporters and show that OpdH is not the major citrate importer; instead, citrate transport primarily relies on the tripartite TctCBA system, which is encoded in the opdH operon. D

SUBMITTER: Underhill SAM 

PROVIDER: S-EPMC9765132 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature