Identification of CD98 as a Novel Biomarker for HIV-1 Permissiveness and Latent Infection.

ABSTRACT: Human immunodeficiency virus type 1 (HIV-1) can integrate viral DNA into host cell chromosomes to establish a long-term stable latent reservoir, which is a major obstacle to cure HIV-1 infection. The characteristics of the HIV-1 latent reservoir have not been fully understood. Here, we identified 126 upregulated plasma membrane proteins in HIV-1 latently infected cells by a label-free liquid chromatography-tandem mass spectrometry analysis. The higher levels of CD98 expression in multiple HIV-1 latently infected cell lines and primary CD4⁺ T cells compared to uninfected cells were further confirmed by quantitative reverse transcription PCR (RT-qPCR) and flow cytometry analyses. In addition, CD98^high CD4⁺ T cells displayed hyper-permissiveness to HIV-1 infec

SUBMITTER: Zhang W 

PROVIDER: S-EPMC9765422 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature