Anaerobic Biohydrogenation of Isoprene by Acetobacterium wieringae Strain Y.

ABSTRACT: Isoprene is a ubiquitously distributed, biogenic, and climate-active organic compound. Microbial isoprene degradation in oxic environments is fairly well understood; however, studies exploring anaerobic isoprene metabolism remain scarce, with no isolates for study available. Here, we obtained an acetogenic isolate, designated Acetobacterium wieringae strain Y, which hydrogenated isoprene to a mixture of methyl-1-butenes at an overall rate of 288.8 ± 20.9 μM day^-1 with concomitant acetate production at a rate of 478.4 ± 5.6 μM day^-1. Physiological characterization demonstrated that isoprene was not utilized in a respiratory process; rather, isoprene promoted acetogenesis kinetically. Bioinformatic analysis and proteomics experiments revealed the expression of candidate

SUBMITTER: Jin H 

PROVIDER: S-EPMC9765523 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature