The Coiled-Coil Forming Peptide (KVSALKE)₅ Is a Cell Penetrating Peptide that Enhances the Intracellular Delivery of Proteins.

ABSTRACT: Protein-based therapeutics have the potential to treat a variety of diseases, however, safe and effective methods for delivering them into cells need to be developed before their clinical potential can be realized. Peptide fusions have great potential for improving intracellular delivery of proteins. However, very few peptides have been identified that can increase the intracellular delivery of proteins, and new peptides that can enhance intracellular protein delivery are greatly needed. In this report, the authors demonstrate that the coiled-coil forming peptide (KVSALKE)₅ (termed K5) can function as a cell penetrating peptide (CPP), and can also complex other proteins that contain its partner peptide E5. It is shown here that GFP and Cas9 fused to the K5 peptide has dramatical

SUBMITTER: Li J 

PROVIDER: S-EPMC9766156 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature