Omics-guided bacterial engineering of Escherichia coli ER2566 for recombinant protein expression.

ABSTRACT: The goal of bacterial engineering is to rewire metabolic pathways to generate high-value molecules for various applications. However, the production of recombinant proteins is constrained by the complexity of the connections between cellular physiology and recombinant protein synthesis. Here, we used a rational and highly efficient approach to improve bacterial engineering. Based on the complete genome and annotation information of the Escherichia coli ER2566 strain, we compared the transcriptomic profiles of the strain under leaky expression and low temperature-induced stress. Combining the gene ontology (GO) enrichment terms and differentially expressed genes (DEGs) with higher expression, we selected and knocked out 36 genes to determine the potential impact of these genes on protein pr

SUBMITTER: Zhou L 

PROVIDER: S-EPMC9767853 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature