Mapping the functional expression of auxiliary subunits of K_Ca1.1 in glioblastoma.

ABSTRACT: Glioblastoma (GBM) is the most aggressive glial tumor, where ion channels, including K_Ca1.1, are candidates for new therapeutic options. Since the auxiliary subunits linked to K_Ca1.1 in GBM are largely unknown we used electrophysiology combined with pharmacology and gene silencing to address the functional expression of K_Ca1.1/β subunits complexes in both primary tumor cells and in the glioblastoma cell line U-87 MG. The pattern of the sensitivity (activation/inhibition) of the whole-cell currents to paxilline, lithocholic acid, arachidonic acid, and iberiotoxin; the presence of inactivation of the whole-cell current along with the loss of the outward rectification upon exposure to the reducing agent DTT collectively argue that K_Ca1.1/β3 complex

SUBMITTER: Feher A 

PROVIDER: S-EPMC9768140 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature