Fluxomics reveals cellular and molecular basis of increased renal ammoniagenesis.

ABSTRACT: The kidney plays a critical role in excreting ammonia during metabolic acidosis and liver failure. The mechanisms behind this process have been poorly explored. The present study combines results of in vivo experiments of increased total ammoniagenesis with systems biology modeling, in which eight rats were fed an amino acid-rich diet (HD group) and eight a normal chow diet (AL group). We developed a method based on elementary mode analysis to study changes in amino acid flux occurring across the kidney in increased ammoniagenesis. Elementary modes represent minimal feasible metabolic paths in steady state. The model was used to predict amino acid fluxes in healthy and pre-hyperammonemic conditions, which were compared to experimental fluxes in rats. First, we found that total renal ammoni

SUBMITTER: Mpabanzi L 

PROVIDER: S-EPMC9768161 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature