Roles of the m6A methyltransferases METTL3, METTL14, and WTAP in pulmonary tuberculosis.

ABSTRACT:

Objective

The aim of the current study was to investigate the contributing role of gene variation and transcription levels among the m6A methyltransferases METTL3, METTL14, and WTAP in pulmonary tuberculosis (PTB).

Methods

A case-control study including 461 PTB patients and 467 normal controls was designed for genotyping. Three SNPs in METTL3 (rs1061027, rs1139130, rs1061026), three SNPs in METTL14 (rs62328061, rs4834698, rs1064034), and two SNPs in WTAP (rs1853259, rs11752345) were genotyped via the SNPscan™ technique. METTL3, METTL14, and WTAP transcription levels were determined in 78 PTB patients and 86 controls via quantitative real-time reverse-transcription PCR.

Results

Frequencies of the METTL14 rs

SUBMITTER: Zhang TP 

PROVIDER: S-EPMC9768477 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature