The Exposure to Human Breast Cancer Cells Altered 14 Post-Translational Modifications of Human Serum Albumin.

ABSTRACT:

Purpose

Serum albumin is in contact with practically all cells in the human body, including tumor cells in cancer patients. The purpose of this study was to explore whether cancer cells affect post-translational modifications (PTMs) of albumin.

Material and methods

Mass spectrometry was used to identify the PTMs. Purified human serum albumin was incubated with human breast cancer cells MDA-MB-231, MDA-MB-468, MCF7, or kept in water or in cell culture media. PTMs which were affected upon exposure of the albumin to cancer cells were identified. Three-dimensional analysis was performed to locate PTMs in albumin.

Results

We report here that an exposure to human breast cancer cells affected post-translational modifications (PTMs) of 14 peptides of human serum albumin (HSA)

SUBMITTER: Kannan S 

PROVIDER: S-EPMC9768621 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature