Biomolecular condensate phase diagrams with a combinatorial microdroplet platform.

ABSTRACT: The assembly of biomolecules into condensates is a fundamental process underlying the organisation of the intracellular space and the regulation of many cellular functions. Mapping and characterising phase behaviour of biomolecules is essential to understand the mechanisms of condensate assembly, and to develop therapeutic strategies targeting biomolecular condensate systems. A central concept for characterising phase-separating systems is the phase diagram. Phase diagrams are typically built from numerous individual measurements sampling different parts of the parameter space. However, even when performed in microwell plate format, this process is slow, low throughput and requires significant sample consumption. To address this challenge, we present here a combinatorial droplet microfluid

SUBMITTER: Arter WE 

PROVIDER: S-EPMC9768726 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature