Construction of an Ordered Transposon Library for Uropathogenic Proteus mirabilis HI4320.

ABSTRACT: Ordered transposon libraries are a valuable resource for many bacterial species, especially those with difficult methods for generating targeted genetic mutations. Here, we present the construction of an ordered transposon library for the bacterial urinary tract pathogen Proteus mirabilis strain HI4320. This library will facilitate future studies into P. mirabilis biology. For large experimental screens, it may be used to overcome bottleneck constraints and avoid biased outcomes resulting from gene length. For smaller studies, the library allows sidestepping the laborious construction of single targeted mutants. This library, containing 18,432 wells, was condensed into a smaller library containing 1,728 mutants. Each selected mutant had a single transposon insertion in an open reading fram

SUBMITTER: Pearson MM 

PROVIDER: S-EPMC9769666 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature